Transfus Med Hemother. 2009;36(3):154-155.

Application of a Multivariant, Caucasian-Specific, Genotyped Donor Panel for Performance Validation of MDmulticard®, ID-System®, and Scangel® RhD/ABO Serotyping

by Christoph Gassner

Christoph Gassner, Esther Rainer, Elfriede Pircher, Lydia Markut, Günther F., Körmöczib Christof, Jungbauerc Dietmar, Wessind Roswitha, Klinghofere Harald, Schennach, Peter Schwind, Diether Schönitzera.



Validations of routinely used serological typing meth- ods require intense performance evaluations typically including large numbers of samples before routine application. However, such evaluations could be improved considering information about the frequency of standard blood groups and their variants.

Study design and methods

Using RHD and ABO population genetic data, a Caucasian-spe- cific donor panel was compiled for a performance comparison of the three RhD and ABO serological typing methods MDmulticard (Medion Diagnostics), ID-System (DiaMed) and ScanGel (Bio-Rad). The final test panel included standard and variant RHD and ABO genotypes, e.g. RhD categories, partial and weak RhDs, RhD DELs, and ABO samples, mainly to interpret weak serological reactivity for blood group A specificity. All samples were from individuals recorded in our local DNA blood group typing database.


For ‘standard’ blood groups, results of performance were clearly interpretable for all three serological methods compared. However, when focusing on specific variant phenotypes, pronounced dif- ferences in reaction strengths and specificities were observed be- tween them.


A genetically and ethnically predefined donor test panel consisting of 93 individual samples only, delivered highly significant results for serological performance comparisons. Such small panels offer impressive representative powers, higher as such based on statistical chances and large numbers only.

PMID: 21113256 PMCID: PMC2980522 DOI: 10.1159/000218364

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