PLoS Pathog. 2010 Apr 29;6(4):e1000891. doi: 10.1371/journal.ppat.1000891.
Complement as an endogenous adjuvant for dendritic cell-mediated induction of retrovirus-specific CTLs.
by
Bánki Z, Posch W, Ejaz A, Oberhauser V, Willey S, Gassner C, Stoiber H, Dittmer U, Dierich MP, Hasenkrug KJ, Wilflingseder D.
Abstract
Previous studies have demonstrated the involvement of complement (C) in induction of efficient CTL responses against different viral infections, but the exact role of complement in this process has not been determined. We now show that C opsonization of retroviral particles enhances the ability of dendritic cells (DCs) to induce CTL responses both in vitro and in vivo. DCs exposed to C-opsonized HIV in vitro were able to stimulate CTLs to elicit antiviral activity significantly better than non-opsonized HIV. Furthermore, experiments using the Friend virus (FV) mouse model illustrated that the enhancing role of complement on DC-mediated CTL induction also occurred in vivo. Our results indicate that complement serves as natural adjuvant for DC-induced expansion and differentiation of specific CTLs against retroviruses.